21 Kas 2024 Perşembe
Regulation of tweedle cuticular protein gene expression at the pre-pupal stage in wing discs of Bombyx mori
Abstract :The present study was undertaken to clarify the regulatory mechanism of Bombyx mori tweedle cuticular protein gene expressed in wing discs at pre-pupal stage with special emphasis to developmental expression and hormonal responsiveness. The tweedle cuticular protein genes BmorCPT2 and BmorCPT3 were selected for the study. The expression of BmorCPT2 was observed from the W3E stage and peaked at pupation when the ecdysteroid titer declined. After pupation suddenly expression decreased. Whereas the transcripts of BmorCPT3 increased from the W3M stage, peaked at W3L, and then decreased rapidly from P0 when the ecdysteroid titer decreasing. BmorCPT3 was induced by the ecdysone pulse, and its expression peaked 18h after transfer to a hormone free medium. BmorCPT2 was also induced by the ecdysone pulse, and its expression peaked 24 h after transfer to a hormone free medium. The peak of BmorCPT2 expression was observed later than that of BmorCPT3 both in vivo and in vitro. Both genes, BmorCPT2 and BmorCPT3 transcripts were not observed after the 20E pulse treatment in the presence of cycloheximide. Transcripts ofBmorCPT3 increased by the addition of 20E in V4 wing discs. On contrary, BmorCPT2 transcript was not observed after the addition of 20E in V4 wing discs which is different from the case of BmorCPT3. The expression profiles of BmorCPT2 are different from those of BmorCPT3. The present findings showed different regulation of tweedle cuticular protein genes at the pre-pupal stage in wing discs of Bombyx mori.
Tweedle cuticular protein, ecdysone, wing disc, gene expression, Bombyx mori