21 Ara 2024 Cumartesi
Study on the sublethal effects of metallic trace element on the antioxidant defence mechanism in fresh water carp
Abstract : Metallic trace elements are essential for normal growth and development of biological tissues in small quantities but when occur in excess concentration they are known to have strong sublethal impact on all the biochemical parameters of eukaryotic experimental models. This hypothesis was investigated in the present study wherein the effects of chromium was examined on the antioxidant defence mechanism in fresh water carp Carassius carassius collected from Dal Lake Srinagar. The carp specimens were subjected to a sublethal döşe of 2.454 ppm i.e. 1/10th of LC50 of potassium dichromate and the oxidative stress was evaluated after 30, 60 and 90 days of treatment. The oxidative biomarkers studied in this experiment were lipid peroxidation (LPO) in addition to antioxidant defence system such as catalase (CAT) and Super oxide dismutase (SOD) activities. The exposure had significant sublethal impact on antioxidant defence mechanism wherein LPO level increased significantly throughout the exposure while as SOD and CAT activities increased up to the 60th day of exposure and later declined from 60th to 90th day (p < 0.05). This surge in the level of LPO and the alteration in the antioxidant defence system could be due to rise in the production of reactive oxygen species (ROS). These results clearly indicate that elemental exposure significantly induced oxidative stress in biological tissue which in turn is associated with tissue damage.