06 Oca 2025 Pazartesi
Primary cell culture development from the heart of Schizothorax plagiostomus
Abstract : Schizothorax plagiostomus, a cold-water fish species, is an essential part of fisheries in the Himalayan and sub-Himalayan regions. However, environmental challenges such as overfishing and habitat disruption have negatively impacted its populations. This study focused on the developing of primary cell cultures from the heart tissue of Schizothorax plagiostomus using the explant culture method. The optimal conditions for cell growth were identified, with cells showing the best proliferation at 24°C in L-15 medium supplemented with 20% fetal bovine serum (FBS). The study also examined the effects of basic fibroblast growth factor (bFGF), which significantly enhanced cell proliferation. The cells exhibited significant growth after 72 hours of implantation, and a confluent monolayer was achieved by day 10, with cells reaching passage 12 after subculturing. The results indicate that temperature, serum concentration, and growth factors play pivotal roles in the successful culture of these cells, making this method a valuable tool for further research in fish virology, transgenesis, and immunological studies. This study can aid in addressing diseases affecting cold-water fishes like Schizothorax plagiostomus.