21 Ara 2024 Cumartesi
Occurrence and prevalence of six honey bee viruses in Hakkari (Turkey) and their genomic divergence
Abstract :A survey study was conducted to establish the occurrence and prevalence
of Black
queen cell virus (BQCV), Deformed
wing virus (DWV), Sacbrood virus
(SBV), Chronic bee paralysis virus (CBPV),
Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV) in traditionally
beekeeping sites of Hakkari province (Turkey). Including the central Hakkari,
the districts of Cukurca, Semdinli and Yuksekova were surveyed to assess the types
of symptoms and the severity of the diseases of honey bees in a total of 90
apiaries. The
presence of BQCV, DWV, SBV and CBPV infections were ascertained
for the first time by RT-PCR. BQCV was found in three sampled districts and was the
most frequently detected virus, found in 32% of bee samples.Less than 24% of
asymptomatic bee samples were positive for DWV, SBV
and CBPV with the infection ratio of 23, 12 and 9%, respectively. The
samples showing deformed wing symptoms were always reacted positive for DWV. During this survey, KBV and IAPV were not detected in
any of tested bee samples. Some double and triple infections were encountered: BQCV+DWV 7 (7.7%),
BQCV+SBV 4 (4.4%), BQCV+CBPV 2 (2.2%), DWV+CBPV 2 (2.2%), DWV+SBV 1 (1.1%) and
DWV+BQCV+SBV 1 (1.1%). The overall incidences of detected viruses (BQCV, DWV,
SBV and CBPV) were lower than the other records of various sites of the world. Based on blast analysis at NCBI
database, the cloned nucleotide sequences of Hakkari’s isolates of BQCV, DWV,
SBV, and CBPV have been showed varied nucleic acid similarities between 77-99%.
Apis mellifera, Hakkari province,
molecular characterization, RNA viruses, RT-PCR