21 Kas 2024 Perşembe
Identification and characterization of alpha-amylase in the rose aphid, Macrosiphum rosae (Linnaeus, 1758) (Hemiptera: Aphididae)
Abstract :Rose aphid, Macrosiphum rosae L. (Hemiptera: Aphididae), is a cosmopolitan pest that can cause severe damage to various members of the Rosaceae family. This pest have various hosts during the year, however, it is most commonly found feeding on roses (Mound & Teulon, 1995).The present study for the first time showed that á-amylase is present in the Macrosiphum rosae L. or rose aphid (Hemiptera: Aphididae). á-Amylases have been found to be active in different insect species (Takagi et al., 1971; Baker, 1991; Kazzazi et al., 2005; Zibaee et al., 2008). Rose aphid á-amylase activity pH was determined to be around 7. Generally, optimal pH is corresponding to the pH prevailing in the midgut from which the enzyme has been extracted so these discrepancies seen in midgut pH are related to the different feeding habits and feeding sources. The optimal temperature for Rose aphid á-amylase activity was 45°C, the enzyme was active over a broad temperature range from 20 to 60°C. Analysis of enzyme homogenates by electrophoresis indicated a band in polyacrylamide gel
á-Amylases, Rose aphid, gut, Macrosiphum rosae L.