21 Ara 2024 Cumartesi
Genetic variability of host populations of the European corn borer, Osrtinia nubilalis (Hübner) (Lepidoptera: Crambidae) in Iran
Abstract :The European corn borer is one of the most important economic pests that could find in all parts of Iran. To study the genetic diversity of O. nubilalis during summer 2013-2014 from first generation in wheat and in second generation from other hosts, 15 individuals of each sex were collected for preliminary analysis. The genomic DNA from European corn borer larvae extracted from host populations of corn, wheat, okra, barley, melon, sugar beet and cocklebur from Ardabil province of Iran using polymerase chain reaction (PCR) and 10 different SSR primers produced 36 polymorphic bands. Nine of the SSR primers showed high variability across the distinct populations with 100 percent polymorphism. Within populations, genetic diversity based on Nei’s gene index ranged from 0.214 to 0.600 at wheat and cocklebur in host populations, respectively. Mean and standard deviation of observed number of alleles were 3.600 and 1.265. Mean of observed hetorozygosity and gene flow for all loci were 0.0857 and 0.020, respectively. Molecular variance analysis showed significant differences within and among populations with groups variance accounted for 13.08 and 97.73, respectively. Using the un-weighted pair-group method analysis, cocklebur population grouped as unique in one cluster while the other populations grouped separately
Genetic diversity, Ostrinia nubilalis, SSR, Hetorozygosity