04 Ara 2024 Çarşamba
Effect of entomopathogenic fungus, Purpureocillium lilacinum (Syn: Paecilomyces lilacinus) on Blatta orientalis and Shelfordella tartara under laboratory conditions
Abstract :Cockroaches
are pests that can threat human health. In this study, the effects of the
entomopathogenic fungus, Purpureocillium
lilacinum TR1 (syn: Paecilomyces
lilacinus), which has been reported from Turkey previously, on the nymphs
of cockroaches [Oriental cockroach (Blatta
orientalis Linnaeus) and Turkestan cockroach (Shelfordella tartara Sauss)] were researched under laboratory
conditions. Experiments were carried out in Petri-dishes with 5 individual
nymphs per replicate, and fungal isolate. Suspensions of P. lilacinum were applied at three different density (106,
107 and 108 cfu ml-1) at spraying applications
the surface of the plates with roaches (after putting in 5 roaches) and without
roaches (before putting in 5 roaches). In the controls, sterile water with
Tween 80 (0.01%) was applied to the surface of the plates with roaches and
without roaches. Each plate contained moistened filter paper on the bottom and
added to the bait (favored bait, banana fruit) placed on the center. The Petri
plates were then placed in a growth chamber maintained at 25±2 °C and 60-65%
R.H. in a dark laboratory. Mortalities were recorded on the 3rd, 6th, 9th, 10th
and 12th days after application. The dead roaches‟ nymphs were examined
under a microscope to determine whether mortality was because of P. lilacinum, and mycosis development
was checked. The results of the study showed that although the effects at
spraying applications the surface of plates with roaches were very high on the
other hand it was very low on spraying applications without roaches. It was
valid for both roach species (B.
orientalis and S. tartara). The
concentrations of 106 and 107 cfu ml-1 at the B. orientalis was found 100% effective;
on the other hand the concentrations of 106 cfu ml-1 at
the S. tartara was found 100%
effective at the same applications 12th
days after application. But all of them were dead at 12th days. The effect of
the other applications (spraying applications the surface of the plates without
roaches) was found below 40% for both roach species 12th days after
application. It is hoped that this first study in laboratory conditions will
serve to the future studies on the control of the pest
Entomopathogenic
fungus, Blatta orientalis, Shelfordella tartara, effect