22 Ara 2024 Pazar
Determination of genetic relationships among three ground spiders (Araneae: Gnaphosidae) by using Rapd-Pcr from Turkey
Abstract :Random amplified polymorphic DNA (RAPD-PCR), was used to
determine three Gnaphosidae species, in the study. RAPD-PCR was carried out by
using fifty random primers each of them consisted of ten base pairs. Fourteen
of these primers gave sufficiently clear and distinguishable bands. Forty eight
samples that represented three Gnaphosidae species, Drassodes lapidosus Walckenaer, 1802, Haplodrassus signifer Koch, 1839 and Nomisia ripariensis Cambridge, 1872, were used for RAPD-PCR and
they were identified by their molecular characteristics. Genetic polymorphism
among these samples was recorded by UPGMA method. The aim of this study was to
achieve genetic marker to clearly differentiate and determine the genetic
variation of these three samples that collected from different locations of Turkey.
Our results showed that RAPD-PCR is an effective, fast and simply alternative
method for identification of the spider species
Drassodes lapidosus, Haplodrassus signifer, Nomisia
ripariensis, RAPD-PCR, genetic polymorphism