Comparing carbohydrate enzymes activity in Eurygaster integriceps Putton (Hemiptera: Scutelleridae), Rhopalosiphum padi L. (Homoptera: Aphididae) and Haplothrips tritici Kurd. (Phlaeothripidae: Thysanoptera) as a complex pest on wheat

Abstract :

Sunn pest (Eurygaster integriceps Putton), Bird cherry-oat aphid (Ropalosiphum padi L.) and wheat thrips (Haplothrips tritici Kurd.) are the most important pests which damage on wheat. They are dependent on their carbohydrate enzymes because of their nutrition diet. The weakness of wheat is caused because of their feeding on plant's carbohydrate materials. In this study the activity of three enzymes including: á-amylase, á and â-glucosidase were assayed in the midgut, salivary glands of sunn pest, the gut of wheat aphid and wheat thrips. Levels of these enzymes activities were different. In sunn pest's midgut the highest activity was found for â-glucosidase and the lowest was for á-amylase whereas in sunn pest salivary gland the highest activity was detected for á-glucosidase and the lowest in á-amylase. In the Aphid gut enzyme activity was different so that the highest activity was detected in á-amylase but the lowest in â-glucosidase. In wheat thrips the highest enzyme activity was detected in â-glucosidase and no á-amylase activity was observed. Comparing these four samples, the alpha amylase activity had the highest level in sunn pest's midgut and the lowest in wheat thrips. The highest activity of á, â-glucosidase enzyme was as the same as á-amylase and in sunn pest midgut was the highest. The lowest activity of á, â- glucosidase was found in wheat thrips and wheat aphid, respectively. So the most important pest is E. integriceps because of its carbohydrate enzymes

Keyword :

á-Amylase, á-Glucosidase, â-Glucosidase, Enzyme assay, Eurygaster integriceps, Ropalosiphum padi, Haplothrips tritici

Author(s) : Seddigh, S., Masoudi-Nejad, A., Tafaghodinia, B., Imani, S.
Downloads : 1833
Published Issue : 2012 Vol. 7 Number 1

2012 Vol. 7 Number 1