Analysis of phylogenetic relationship and genetic diversity of Aedes (Diptera: Culicidae) mosquito species based on mitochondrial genes

Abstract :

Aedes mosquitoes, specifically Aedes aegypti and Aedes albopictus are the most potent vector of multiple pathogens. The emergence of molecular tools encourages new species-specific genetic-based approaches to reduce the burden of most vector-borne diseases. Phylogenetic relationships and population genetic study of Aedes aegypti and Aedes albopictus were performed using mitochondrial COI, Cytb gene in the present study. The acquired COI and Cytb sequences of Aedes mosquitoes were subjected to phylogenetic analysis. The sequences were aligned through the ClustalW algorithm and proceeded for estimation of the transition/transversion bias, pairwise genetic divergence and phylogenetic analysis. The frequency of gene flow, genetic distances among haplotypes (Fst) and the number of migration (Nm) were calculated among the studied population. Based on the nucleotide differences, a haplotype network was constructed to establish the genealogical relationship between haplotypes. In this study, both COI & Cytb dependent analysis strongly supports the positioning of Aedes aegypti and Aedes albopictus by forming clusters with their respective group. Population genetic study has illustrated the occurrence of genetic differentiation within the population and the frequent exchange of genes among the Indian and Malaysian Aedes mosquito population. The haplotype network study indicated that the Indian population displays a certain degree of genetic differences among each Aedes mosquito species.

Keyword :

Molecular phylogeny, genetic divergence, DNA barcoding, Aedes mosquito, India

Author(s) : Panda, D., Patro, A., Barik, T. K.
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Published Issue : 2022 Vol. 17 Number 2

2022 Vol. 17 Number 2