Laboratory evaluation of ethanolic and methanolic extracts of Ocimum gratissimum against larva of Anopheles gambiae and non-target organisms

Abstract : Methanolic and ethanolic extracts of Ocimum gratissimum leaves were evaluated for larvicidal activity against larva of Anopheles gambiae and non target organisms in the laboratory. Photochemical analysis of leaf showed presence of tannins, alkaloids, flavonoid, cyanogenic glycosides, cardiac glycosides and saponin. The organisms were exposed to different test concentrations ranging from 20mg/ml to 100mg/ml for 96hours. Larvae of A. gambiae were susceptible to the extracts. Ethanolic extracts of the leaf was more active with LC50 and LC90 of 60.9mg/ml and 464.4 mg/ml respectively while the LC50 and LC90 of methanolic extracts of leaf were 73.6mg/ml and 1021mg/ml respectively. The extracts exhibited insect growth regulating activity and were found to be safe to non target organisms. Results showed that larvicidal effect of the methanolic extract can be compared to that of neem leaf extracts
Keyword : Ocimum gratissimum leaves, methanolic and ethanolic extract, larvicidal activity, Anopheles gambiae, non target organisms
Author(s) : , P. U. Ofoegbu, N. E. Onyedineke, C. Nwokeji, N. G. Esie, N. Isibor
Downloads : 1266

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