Characterization of á-amylase in the alimentary canal of Naranga aenescens Moore (Lepidoptera: Noctuidae), the Rice green caterpillar

Abstract : The á-amylases in whole gut of Naranga aenescens (Lepidoptera: Noctuidea) were isolated and characterized by using spectrophotometrically assays. The results showed the existence of the enzyme with a high activity in the gut so that the optimal pH and temperature were evaluated 10 and 30 °C, respectively. The activity of á-amylase was inhibited by NaCl and CaCl2 and increased or remained no effect by other used compounds. Determination of the band on the Native-PAGE electrophoresis showed a single isozyme of á-amylase in the gut N. aenescens. Study on digestive enzymes of insects is one of the new and winning area to reach a safe and effective way to decrease the damages of the pest on agricultural products. Also, determination of different specific inhibitors are necessary to reach a real and reliant results.
Keyword : á-amylases, Rice green caterpillar, gut
Author(s) : , A. R. Bandani, F. Maleki, S. Rahmani, M. Fazeli-Dinan
Downloads : 2300


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